Title
Topic
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Advances in imaging amphibian regeneration
This book chapter (from “Salamanders: Methods and Protocols”) provides an alternative protocol to the in situ hybridization of amphibians. While this protocol “has been utilized for decades in axolotls, it has been challenging to implement consistently across tissues.” The authors here present an approach combining a hybridized chain reaction (HCR) with fluorescence in situ hybridization (FISH), providing a method with “a considerably higher signal to background” noise ratio. See the full list of authors and read this book chapter, “Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue,” at the publisher’s page.
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Raising (regenerative) axolotls for experimental uses
Mexican axolotls are capable of regenerating “amputated limbs and injured body parts,” and their study is valuable to both stem cell and regeneration research. From “Salamanders: Methods and Protocols”, this book chapter by professor James Monaghan and PhD. Anastasia Yandulskaya presents the conditions for raising lab-healthy axolotls, how to breed them, and how to maintain their environment. Read this book chapter, “Establishing a New Research Axolotl Colony,” at the publisher’s page.